ANNOgesic is the swiss army knife for RNA-Seq based annotation of bacterial/archaeal genomes. It is a modular, command-line tool that can integrate different types of RNA-Seq data based on dRNA-Seq (differential RNA-Seq) or RNA-Seq protocols that inclusde transcript fragmentation to generate high quality genome annotations. It can detect more than 20 genomic features. The tool was heavily tested with several RNA-Seq data set from bacterial as well as archaeal samples.